2MSc, Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran DOI : 10.37845/ret.vit.2024.33.8 Purpose: Age-related macular degeneration (AMD) is a progressive disease resulting in loss of vision. One of the important factors in AMD disease is oxidative stress. Excessive accumulation of ROS and the inability of the antioxidant defense system to neutralize it can contribute to the development of lesions. One of the antioxidant enzymes is glutathione peroxidase. The aim of this study was to investigate the relation between genetic polymorphisms of GPX1 C594T and GPX4 C718T with the risk of AMD disease.
Materials and Methods: In this case-control study, 122 AMD patients and 122 healthy controls group matched for gender and age. Genotyping of GPX1 and GPX4 were done by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method.
Results: There was no significant relationship between allele and genotype frequency in the genetic polymorphisms of GPX1 C594T (rs1050450) and GPX4 C718T (rs713041) and susceptibility to AMD. The association between smoking and AMD was found in this study (OR = 2.165, CI = 1.145-4.092, P = 0.017). Also, there was a significant correlation between the number of patients who were working outside the room (OR = 2.067, CI = 1.168-3.659, P = 0.013).
Conclusions: The current study suggested that the GPX1 C594T (rs1050450) and GPX4 C718T (rs713041) polymorphisms are not predisposing to AMD.
Keywords : Age related macular degeneration (AMD), GPX1, GPX4, Polymorphism